He Y, Thong P S, Lee T, Leong S K, Shi C Y, Wong P T, Yuan S Y, Watt F
Department of Surgery, National University of Singapore, Singapore.
Brain Res. 1996 Sep 30;735(1):149-53. doi: 10.1016/0006-8993(96)00313-7.
The trace elemental concentrations, including iron, in the substantia nigra (SN) of a 6-OHDA induced rat model of Parkinson's disease were measured using nuclear microscopy. Only rats that exhibited amphetamine induced rotation of more than 7 turns/min were used. The results showed that the iron levels were significantly increased in the 6-OHDA lesioned SN, compared with the intact contralateral SN, and the SN of normal control rats injected with ascorbic acid, which showed no significant difference in iron levels between injected and non-injected sides. In both 6-OHDA lesioned and ascorbic acid injected SN, there were no alterations in the levels of calcium, magnesium, copper and zinc. In the 6-OHDA lesioned SN there was an almost complete loss of tyrosine hydroxylase positive cells in the SN. These results suggested that the 6-OHDA induced dopaminergic cell death may be related to the increased iron.
利用核显微镜测量了6-羟基多巴胺诱导的帕金森病大鼠模型黑质（SN）中的微量元素浓度，包括铁含量。仅使用了表现出安非他明诱导旋转超过7转/分钟的大鼠。结果显示，与完整的对侧黑质以及注射了抗坏血酸的正常对照大鼠的黑质相比，6-羟基多巴胺损伤的黑质中铁水平显著升高，而注射了抗坏血酸的正常对照大鼠注射侧和未注射侧的铁水平无显著差异。在6-羟基多巴胺损伤的黑质和注射了抗坏血酸的黑质中，钙、镁、铜和锌的水平均无变化。在6-羟基多巴胺损伤的黑质中，黑质中酪氨酸羟化酶阳性细胞几乎完全丧失。这些结果表明，6-羟基多巴胺诱导的多巴胺能细胞死亡可能与铁含量增加有关。
