Fang Y, Zhang J Z, Hou J Y, Lu H, Dovichi N J
Department of Chemistry, University of Alberta, Edmonton, Canada.
Electrophoresis. 1996 Sep;17(9):1436-42. doi: 10.1002/elps.1150170907.
We present the mobility of DNA sequencing fragments as a function of temperature in 5%T, 0%C noncross-linked polyacrylamide and at an electric field of 150 V/cm. The mobility of the fragments follows the Arrhenius equation. The activation energy for migration of a fragment through the polymer decreases monotonically with fragment length. The data were also analyzed in terms of the biased reptation model; the onset of biased reptation with stretching occurs for longer fragments as the temperature increase. These results are quite different from those observed for separation of DNA in cross-linked gels and represent a fundamental difference in the physics of the cross-linked and noncross-linked polyacrylamide.
我们展示了在5%T、0%C的非交联聚丙烯酰胺中，温度为变量，电场为150 V/cm时DNA测序片段的迁移率。片段的迁移率遵循阿累尼乌斯方程。片段在聚合物中迁移的活化能随片段长度单调降低。数据也依据有偏蠕动模型进行了分析；随着温度升高，对于较长片段，会出现因拉伸导致的有偏蠕动。这些结果与在交联凝胶中分离DNA时观察到的结果截然不同，代表了交联和非交联聚丙烯酰胺在物理性质上的根本差异。
