Rojas L X, McDowell L R, Cousins R J, Martin F G, Wilkinson N S, Johnson A B, Velasquez J B
Animal Science Department, University of Florida, Gainesville 32611, USA.
J Trace Elem Med Biol. 1996 Sep;10(3):139-44. doi: 10.1016/S0946-672X(96)80023-X.
A study was conducted to compare bioavailability, interactions and retention of different sources of Zn and Cu fed to rats. Sixty-three male CD rats were fed individually a purified diet and deionized water ad libitum. The nine treatments included were all combinations of three Zn (ZnMet, ZnLys, ZnSO4) and three Cu (CuLys, CuSO4, CuO) sources added to the basal diet at 30 mg/kg of Zn and 6 mg/kg of Cu forming a 3' 3 factorial experiment. After the four-week supplementation phase, four randomly selected rats from each treatment were sacrificed (Phase 1). The remaining rats were fed the purified, unsupplemented diet for an additional week (Phase 2) and then sacrificed. Mineral (Zn and Cu) concentrations were determined in plasma, liver, kidney, bone and muscle, and metallothionein (MT) content was determined in liver and kidney. Plasma Cu concentrations were lower (p < 0.05) for CuO-than CuSO4- and CuLys-supplemented rats. Bone Zn concentrations were higher (p < 0.05) for CuLys-than for CuO-supplemented rats. In all tissues where Cu was measured, CuO was the lowest (p < 0.05) available source of Cu. Furthermore, in muscle, CuSO4-supplemented rats had higher (p < 0.05) Cu concentrations than CuLys-supplemented rats. Kidney MT concentrations followed the same pattern as Cu concentrations, with CuO-fed rats having the lowest (p < 0.05) MT concentrations. Plasma Cu concentrations of depleted rats were lower (p < 0.05) for CuO-than CuLys-supplemented rats. Kidney Zn concentrations were lower (p < 0.05) for CuSO4-than for CuO-supplemented rats after depletion. In liver, CuO supplemented rats had the lowest (p < 0.05) Cu concentration. Copper oxide was less available than CuLys and CuSO4 when added in adequate dietary levels. However, organic (ZnMet and ZnLys) and inorganic (ZnSO4) sources of Zn were similar.
开展了一项研究，以比较给大鼠喂食不同来源的锌和铜后的生物利用度、相互作用及留存情况。63只雄性CD大鼠单独随意喂食纯化日粮和去离子水。所包含的9种处理方式为，在基础日粮中添加三种锌（蛋氨酸锌、赖氨酸锌、硫酸锌）和三种铜（赖氨酸铜、硫酸铜、氧化铜）来源的所有组合，添加量为每千克锌30毫克、每千克铜6毫克，构成一个3×3析因试验。在为期四周的补充阶段后，从每个处理组中随机挑选4只大鼠处死后（第1阶段）。其余大鼠再喂食一周纯化的未补充日粮（第2阶段），然后处死后。测定血浆、肝脏、肾脏、骨骼和肌肉中的矿物质（锌和铜）浓度，并测定肝脏和肾脏中的金属硫蛋白（MT）含量。喂食氧化铜的大鼠血浆铜浓度低于喂食硫酸铜和赖氨酸铜的大鼠（p<0.05）。喂食赖氨酸铜的大鼠骨骼锌浓度高于喂食氧化铜的大鼠（p<0.05）。在所有测量铜的组织中，氧化铜是最低的（p<0.05）可利用铜源。此外，在肌肉中，喂食硫酸铜的大鼠铜浓度高于喂食赖氨酸铜的大鼠（p<0.05）。肾脏MT浓度与铜浓度的模式相同，喂食氧化铜的大鼠MT浓度最低（p<0.05）。耗尽后，喂食氧化铜的大鼠血浆铜浓度低于喂食赖氨酸铜的大鼠（p<0.05）。耗尽后，喂食硫酸铜的大鼠肾脏锌浓度低于喂食氧化铜的大鼠（p<0.05）。在肝脏中，喂食氧化铜的大鼠铜浓度最低（p<0.05）。当以适当的日粮水平添加时，氧化铜的可利用性低于赖氨酸铜和硫酸铜。然而，锌的有机（蛋氨酸锌和赖氨酸锌）和无机（硫酸锌）来源相似。
