Seale T W, Niekrasz I, Garrett K M
Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Neuroreport. 1996 Jul 29;7(11):1803-8. doi: 10.1097/00001756-199607290-00023.
This study extends the pharmacological characterization of a novel quantitative murine behavioral method, the mirrored chamber aversion assay, which appears to be selectively sensitive to anxiolytic agents. Behavioral effects of acute diazepam administration were compared with those of the 5-HT1A anxiolytic buspirone and those of ethanol in C57BL/6J mice. These known anxiolytics produced a dose-dependent reduction in avoidance behavior of large magnitude, as evidenced by statistically significant decreases in latency to enter and increases in time spent in the mirrored chamber. Anxiolytic-like effects of these compounds in the mirrored chamber assay differed from those observed by the elevated plus-maze method. The behavioral effects of the test compounds were not due to alteration of locomotor activity. These findings indicate that the murine mirrored chamber assay responds to several agents known to be anxiolytic in man but differs from the plus-maze in the pharmacological spectrum of the anxiolytics to which it is sensitive.
本研究扩展了一种新型定量小鼠行为学方法——镜像箱厌恶试验的药理学特征，该试验似乎对抗焦虑药具有选择性敏感性。在C57BL/6J小鼠中，比较了急性给予地西泮、5-HT1A抗焦虑药丁螺环酮和乙醇的行为效应。这些已知的抗焦虑药产生了幅度较大的剂量依赖性回避行为减少，进入镜像箱潜伏期的统计学显著降低以及在镜像箱中停留时间的增加证明了这一点。这些化合物在镜像箱试验中的抗焦虑样效应与在高架十字迷宫法中观察到的不同。受试化合物的行为效应并非由于运动活性的改变。这些发现表明，小鼠镜像箱试验对几种已知对人类有抗焦虑作用的药物有反应，但在其敏感的抗焦虑药药理学谱方面与十字迷宫试验不同。
