Kubota M, Landgraf R, Wotjak C T
Max Planck Institute of Psychiatry, Munich, Germany.
Neuroreport. 1996 Aug 12;7(12):1933-6. doi: 10.1097/00001756-199608120-00013.
The release of arginine vasopressin (AVP) within the suprachiasmatic nucleus (SCN) of anaesthetized male rats was monitored by microdialysis. Dialysis of the nucleus with 56 mM K+ solution (to 400%) as well as with 1 M NaCl-hypertonic medium (to 530%) triggered a significant increase in intra-SCN release of AVP (p < 0.01). To investigate whether endogenous AVP influences its own release, we administered in a second experiment the combined V1/V2 receptor antagonist d(CH2)5[D-Tyr(Et)2,Val4]AVP directly into the dialysed area. Compared with vehicle-treated controls, the antagonist had no effects on intranuclear release of AVP during basal or stimulated conditions. Thus, in contrast to recent observations in the supraoptic nucleus, we found no indications for autoregulatory mechanisms of AVP release within the SCN.
通过微透析监测麻醉雄性大鼠视交叉上核（SCN）中精氨酸加压素（AVP）的释放。用56 mM K⁺溶液（至400%）以及1 M NaCl高渗培养基（至530%）对该核进行透析，引发了SCN内AVP释放的显著增加（p < 0.01）。为了研究内源性AVP是否影响其自身释放，在第二个实验中，我们将V1/V2受体联合拮抗剂d(CH2)5[D-Tyr(Et)2,Val4]AVP直接注入透析区域。与用赋形剂处理的对照组相比，该拮抗剂在基础或刺激条件下对核内AVP释放均无影响。因此，与最近在视上核中的观察结果相反，我们未发现SCN内AVP释放存在自调节机制的迹象。
