Ujike H, Akiyama K, Kuroda S
Department of Neuropsychiatry, Okayama University Medical School, Japan.
Neuroreport. 1996 Aug 12;7(12):2036-40. doi: 10.1097/00001756-199608120-00037.
Changes in transcription of Gs alpha, Gi2 alpha and Go alpha proteins after repeated methamphetamine administration were investigated using in situ hybridization. Rats received daily injections of methamphetamine (4 mg kg-1) for 14 days. Four hours after the last injection, Go alpha mRNA showed an increase of 16.6-31.6% in CA1, CA3 and dentate gyrus of the hippocampus. Gi alpha mRNA level increased by 24.2% in CA1, while Gs alpha mRNA remained unaffected. Go alpha and Gi alpha mRNA returned to basal levels after 48 h abstinence. The expression of these G alpha mRNA in other brain regions, including substantia nigra, ventral tegmentum area, striatum, accumbens and cortices, was unchanged. The increase in Go alpha and Gi alpha mRNAs restricted only to the hippocampus after repeated methamphetamine administration may be involved in the development of dependence rather than behavioral sensitization.
采用原位杂交技术研究了反复给予甲基苯丙胺后Gsα、Gi2α和Goα蛋白转录的变化。大鼠每天注射甲基苯丙胺（4mg/kg-1），持续14天。末次注射后4小时，海马CA1、CA3和齿状回中GoαmRNA增加了16.6-31.6%。CA1区GiαmRNA水平增加了24.2%，而GsαmRNA未受影响。戒断48小时后，Goα和GiαmRNA恢复到基础水平。这些GαmRNA在包括黑质、腹侧被盖区、纹状体、伏隔核和皮质在内的其他脑区的表达未发生变化。反复给予甲基苯丙胺后，Goα和GiαmRNA的增加仅局限于海马，这可能与成瘾的形成有关，而非行为敏化。
