某些阳离子对致病疫霉游动孢子形成和感染性的影响。2. 铜、硼、钴、锰、钼和锌离子的影响

Effects of certain cations on the formation and infectivity of Phytophthora zoospores. 2. Effects of copper, boron, cobalt, manganese, molybdenum, and zinc ions.

作者信息

Halsall D M

出版信息

Can J Microbiol. 1977 Aug;23(8):1002-10. doi: 10.1139/m77-149.

Abstract

The effects of copper, boron, cobalt, manganese, molybdenum, and zinc on the production of zoosporangia by P. cinnamomi and P. drechsleri in the presence of favourable concentrations of calcium, magnesium, potassium, and iron were investigated. Copper ions were the most effective in reducing the numbers of zoosporangia formed by both fungal species. Molybdenum was also slightly inhibitory. Total inhibition of mycelial growth occurred between 1 and 5 X 10(-5) M Cu2+ whereas total inhibition of sporangial formation occurred between 1 and 5 X 10(-7) M Cu2+. At copper concentrations between 10(-5) M and 5 X 10(-7) M, many P. drechsleri zoosporangia were abnormal in appearance and nonviable. Infection of eucalypt cotyledons by P. drechsleri zoospores was inhibited by 10(-6) M Cu2+ but this inhibition was reversed by EDTA (10(-4) M). There was no evidence for interaction between Cu2+ and Ca2+, Mg2+, K+, or Fe2+ present in the solutions used in the axenic production of zoospores. Preliminary pot trials indicated that CuSO4 had a protective action for safflower seedlings to infection by P. drechsleri when CuSO4 was applied as a dilute solution over the infection period.

摘要

研究了在存在适宜浓度的钙、镁、钾和铁的情况下，铜、硼、钴、锰、钼和锌对樟疫霉和德氏腐霉游动孢子囊产生的影响。铜离子在减少两种真菌形成的游动孢子囊数量方面最为有效。钼也有轻微的抑制作用。在1至5×10⁻⁵ M Cu²⁺之间菌丝体生长完全受到抑制，而在1至5×10⁻⁷ M Cu²⁺之间孢子囊形成完全受到抑制。在铜浓度介于10⁻⁵ M和5×10⁻⁷ M之间时，许多德氏腐霉游动孢子囊外观异常且无活力。10⁻⁶ M Cu²⁺抑制了德氏腐霉游动孢子对桉树叶柄的侵染，但这种抑制作用可被EDTA（10⁻⁴ M）逆转。在用于无菌生产游动孢子的溶液中，没有证据表明Cu²⁺与Ca²⁺、Mg²⁺、K⁺或Fe²⁺之间存在相互作用。初步盆栽试验表明，当在感染期以稀溶液形式施用硫酸铜时，硫酸铜对红花幼苗受德氏腐霉侵染具有保护作用。