Lawler G C, Weber D J
Can J Microbiol. 1977 Aug;23(8):1018-25. doi: 10.1139/m77-151.
A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicates three metabolic phases. The first is associated with spore germination; the second, trophophase, with growth; and the third, idiophase, with sporulation. Synchronous sporulation and the occurrence of distinct metabolic phases are taken as indications of fairly uniform cell development in the surface cultures. A photographic method of estimating large numbers of fruiting structures on semisolid medium is also described.
描述并评估了一种在费氏根霉液体表面培养的分化期发育过程中获得同步孢子形成的方法，该方法用于研究与无性孢子形成相关的代谢。在这个系统中，生长和孢子形成有效地分开。呼吸模式表明有三个代谢阶段。第一个阶段与孢子萌发有关；第二个阶段，营养期，与生长有关；第三个阶段，分化期，与孢子形成有关。同步孢子形成和不同代谢阶段的出现被视为表面培养物中细胞相当均匀发育的指标。还描述了一种估计半固体培养基上大量子实体结构的摄影方法。
