Wakugawa M, Nakagawa H, Yamada N, Tamaki K
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Int Arch Allergy Immunol. 1996;111 Suppl 1:5-11. doi: 10.1159/000237404.
To investigate the process of eosinophil recruitment in atopic dermatitis (AD), patch testing with crude dust-mite allergens was performed on normal-appearing skin of 9 adult AD patients with high levels of mite-specific IgE antibodies. Positive reactions were observed in 6 AD subjects, whereas 0 of 7 control subjects showed positive reactions to mite allergens. Positive reaction sites were biopsied chronologically and studied histologically and immunohistochemically. Eosinophils were seen in postcapillary venules in the dermis at 2 h, followed by infiltration of eosinophils at 6 h which peaked at 24 and 48 h. In the epidermis, eosinophilic spongiosis was seen at 48 h. Positive reactions against eosinophil granule proteins were observed in connective tissues as well as on eosinophils. Almost all infiltrating eosinophils were positive for BMK-13 (an antibody against major basic protein); half of them were positive for EG2 (an antibody against eosinophil cationic protein). With regard to adhesion molecules, expression of E-selectin and intercellular adhesion molecule-1 endothelial cells was up-regulated as infiltrating eosinophils increased in number. These findings suggest that eosinophil transmigration from endothelial cells and eosinophil degranulation play important roles in initiating early AD lesions induced by transepidermal mite allergen permeation.
为研究特应性皮炎（AD）中嗜酸性粒细胞募集过程，对9名成年AD患者外观正常且螨特异性IgE抗体水平高的皮肤进行粗制尘螨变应原斑贴试验。6名AD受试者出现阳性反应，而7名对照受试者中0人对螨变应原呈阳性反应。对阳性反应部位按时间顺序进行活检，并进行组织学和免疫组织化学研究。2小时时在真皮毛细血管后微静脉中可见嗜酸性粒细胞，随后在6小时时嗜酸性粒细胞浸润，在24小时和48小时达到峰值。在表皮中，48小时时可见嗜酸性海绵形成。在结缔组织以及嗜酸性粒细胞上观察到针对嗜酸性粒细胞颗粒蛋白的阳性反应。几乎所有浸润的嗜酸性粒细胞对BMK - 13（一种抗主要碱性蛋白的抗体）呈阳性；其中一半对EG2（一种抗嗜酸性粒细胞阳离子蛋白的抗体）呈阳性。关于黏附分子，随着浸润嗜酸性粒细胞数量增加，E - 选择素和细胞间黏附分子 - 1内皮细胞的表达上调。这些发现表明，嗜酸性粒细胞从内皮细胞的迁移和嗜酸性粒细胞脱颗粒在由经表皮螨变应原渗透诱导的早期AD病变起始中起重要作用。
