Kishimoto S, Shimazu W, Izumi T, Shimizu T, Fukuda T, Makino S, Sugiura T, Waku K
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Int Arch Allergy Immunol. 1996;111 Suppl 1:63-5. doi: 10.1159/000237419.
The effects of interleukin-3 (IL-3), IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) on the surface expression of platelet-activating factor (PAF) receptor on eosinophils was examined using a PAF receptor binding assay. Specific binding of [3H]WEB 2086, a specific ligand for PAF receptor, to peripheral blood eosinophils obtained from healthy individuals was significantly increased when the cells were cultured with IL-3, IL-5, or GM-CSF for 12 h, compared with untreated cells. The PAF-induced increase in intracellular calcium ion concentration in eosinophils was markedly augmented by exposure to IL-5 for 12 h. These results suggest that these cytokines modulate the responsiveness of eosinophils to PAF through enhancement of PAF receptor expression on the cell surface.
使用血小板活化因子（PAF）受体结合试验，检测了白细胞介素-3（IL-3）、IL-5和粒细胞-巨噬细胞集落刺激因子（GM-CSF）对嗜酸性粒细胞表面PAF受体表达的影响。与未处理的细胞相比，当从健康个体获得的外周血嗜酸性粒细胞与IL-3、IL-5或GM-CSF培养12小时时，PAF受体特异性配体[3H]WEB 2086与这些细胞的特异性结合显著增加。暴露于IL-5 12小时后，PAF诱导的嗜酸性粒细胞内钙离子浓度增加明显增强。这些结果表明，这些细胞因子通过增强细胞表面PAF受体的表达来调节嗜酸性粒细胞对PAF的反应性。
