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激肽释放酶及类激肽释放酶蛋白酶:通过色谱法和电泳法进行纯化与测定

Kallikrein and kallikrein-like proteinases: purification and determination by chromatographic and electrophoretic methods.

作者信息

Raspi G

机构信息

Dipartimento di Chimica e Chimica Industriale dell'Università and Istituto di Chimica Analitica Strumentale del C.N.R. Pisa, Italy.

出版信息

J Chromatogr B Biomed Appl. 1996 Sep 20;684(1-2):265-87. doi: 10.1016/0378-4347(96)00144-2.

Abstract

Kallikreins and kallikrein-like enzymes make up a family of serine proteinases present in tissues and body fluids of mammals and in some snake venoms. This review deals with the procedures of purification, detection and determination of these enzymes by chromatographic and electrophoretic methods. The procedures are reported in tables, described and discussed with the aim of illustrating the state-of-the-art of research in the field.

摘要

激肽释放酶和类激肽释放酶构成了一类丝氨酸蛋白酶家族,存在于哺乳动物的组织和体液以及某些蛇毒中。本综述涉及通过色谱和电泳方法对这些酶进行纯化、检测和测定的过程。这些过程列于表格中,并进行了描述和讨论,旨在阐明该领域的研究现状。

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