Teshima S, Natori T, Nakamura K, Aizawa M, Sakashita S
Hokkaido Igaku Zasshi. 1979 Jan;54(1):31-8.
A study on the experimentally induced yolk sac tumor in the rat was made in special regard to the characteristics and origin of tumor cells. Pregnant rats which fetuses were removed on the 12th day of gestation, developed tumors derived from the fetal membranes left outside the uterus, which were composed of differentiated teratomas and yolk sac tumors. Serial observation of the oncogenesis revealed that an early lesion of the yolk sac tumor appeared in a nodule found as early as 3 weeks after the fetectomy and production of alpha-phetoprotein (AFP) was observed histochemically in the tumor cells 5 weeks after the fetectomy. A cultured cell line established after cloning from the transplantable yolk sac tumor which had been induced similarly and converted into ascitic form was also investigated. Light and electron microscopic studies on both induced tumors and the cultured cells indicated a similarity of AFP producing tumor cells with parietal yolk sac cells and of PAS-positive hyaline-like substance with the Reichert membrane. It is reasonable to conclude that the yolk sac tumor observed is regarded as a parietal yolk sac carcinoma described by Pierce. Histogenesis of the tumor is also discussed.
对大鼠实验性诱导的卵黄囊瘤进行了一项研究，特别关注肿瘤细胞的特征和起源。在妊娠第12天摘除胎儿的怀孕大鼠，会形成源自子宫外残留胎膜的肿瘤，这些肿瘤由分化型畸胎瘤和卵黄囊瘤组成。对肿瘤发生的连续观察表明，卵黄囊瘤的早期病变最早在摘除胎儿后3周发现的一个小结节中出现，摘除胎儿后5周在肿瘤细胞中通过组织化学方法观察到甲胎蛋白（AFP）的产生。还对从类似诱导并转化为腹水形式的可移植卵黄囊瘤克隆后建立的细胞系进行了研究。对诱导肿瘤和培养细胞的光镜和电镜研究表明，产生AFP的肿瘤细胞与壁层卵黄囊细胞相似，PAS阳性透明样物质与赖歇特膜相似。可以合理地得出结论，观察到的卵黄囊瘤被认为是皮尔斯描述的壁层卵黄囊癌。还讨论了肿瘤的组织发生。
