Pijnenborg R, D'Hooghe T, Vercruysse L, Bambra C
Department of Obstetrics and Gynaecology, Katholieke Universiteit Leuven, Belgium.
J Med Primatol. 1996 Aug;25(4):272-81. doi: 10.1111/j.1600-0684.1996.tb00210.x.
Biopsies of placentas (n = 21), placental bed (n = 17) and decidua (n = 26) of various gestation periods (30 to 140 days) were used to study trophoblast invasion in the baboon. Application of immunohistochemical staining for cytokeratin allowed proper identification of trophoblast. Earlier reports showing restricted trophoblast invasion in this species were confirmed by the finding that endovascular trophoblast was present in only one third of biopsies containing spiral arteries. Moreover, immunostaining for cytokeratin revealed that in several arteries only a few isolated trophoblastic cells were present, while the vessel had not undergone the normal physiological change. Trophoblast invasion could only be detected within decidual, but not in myometrial, segments of spiral arteries. Interstitial trophoblast invasion was very limited and multinuclear giant cells were absent. Immunohistochemical staining suggested a contribution of laminin to the fibrinoid deposition within the physiologically changed spiral arteries, while fibronectin was present intracellularly in the invaded trophoblast. Because of differences in the trophoblast invasion pattern, the baboon cannot be regarded as a satisfactory experimental model to explore results of inadequate endovascular trophoblast invasion which, in the human, leads to pregnancy complications such a preeclampsia.
利用不同孕期（30至140天）的胎盘（n = 21）、胎盘床（n = 17）和蜕膜（n = 26）活检组织来研究狒狒的滋养层细胞浸润情况。应用细胞角蛋白免疫组织化学染色可准确识别滋养层细胞。早期报告显示该物种滋养层细胞浸润受限，本研究发现仅三分之一含有螺旋动脉的活检组织中存在血管内滋养层细胞，这证实了早期报告的结果。此外，细胞角蛋白免疫染色显示，在一些动脉中仅存在少数孤立的滋养层细胞，而血管并未发生正常的生理变化。仅在螺旋动脉的蜕膜段而非肌层段可检测到滋养层细胞浸润。间质滋养层细胞浸润非常有限，且不存在多核巨细胞。免疫组织化学染色表明，层粘连蛋白对生理改变的螺旋动脉内的纤维蛋白样沉积有一定作用，而纤连蛋白存在于侵入的滋养层细胞内。由于滋养层细胞浸润模式存在差异，狒狒不能被视为一个理想的实验模型，用以探究血管内滋养层细胞浸润不足在人类中导致诸如先兆子痫等妊娠并发症的相关结果。
