Yoshimoto T, Houkin K, Abe H
Department of Neurosurgery, Hokkaido University School of Medicine, Sapporo.
Neurol Med Chir (Tokyo). 1996 Feb;36(2):67-71. doi: 10.2176/nmc.36.67.
Activated leukocytes may be involved in the reperfusion injury of the brain. The expression of intracellular adhesion molecule-1 (ICAM-1) (CD54) and lymphocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) are important for the interaction of activated leukocytes with the brain. Therefore, the expression of ICAM-1 in the cerebral vessels and LFA-1 on leukocytes were examined after reperfusion in a rat four-vessel occlusion model. Model rats underwent reperfusion (15, 30, and 60 min, and 6, 12, and 24 hrs) following 30 minutes of forebrain ischemia. Immunohistological staining for ICAM-1 and LFA-1 was performed in each subgroup. ICAM-1 expression increased after 1-hour reperfusion and persisted on the cerebral microvessels in both the subcortical region and the basal ganglia. Leukocytes stained by LFA-1 were observed in the capillary vessels after 6-hour reperfusion. Increased expression of ICAM-1 and LFA-1 were induced by reperfusion, and this may be important in reperfusion injury of the brain.
活化的白细胞可能参与脑的再灌注损伤。细胞间黏附分子-1（ICAM-1）（CD54）和淋巴细胞功能相关抗原-1（LFA-1）（CD11a/CD18）的表达对于活化的白细胞与脑的相互作用很重要。因此，在大鼠四动脉闭塞模型再灌注后，检测了脑血管中ICAM-1的表达以及白细胞上LFA-1的表达。模型大鼠在前脑缺血30分钟后进行再灌注（15、30和60分钟，以及6、12和24小时）。对每个亚组进行ICAM-1和LFA-1的免疫组织化学染色。再灌注1小时后ICAM-1表达增加，并在皮质下区域和基底神经节的脑微血管中持续存在。再灌注6小时后，在毛细血管中观察到被LFA-1染色的白细胞。再灌注诱导了ICAM-1和LFA-1表达的增加，这可能在脑的再灌注损伤中起重要作用。
