Shiga Y, Kametani S, Mizuno H, Akanuma H
Department of Life Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku.
J Biochem. 1996 Jan;119(1):173-9. doi: 10.1093/oxfordjournals.jbchem.a021205.
The cyclic polyol 1,5-anhydro-D-glucitol (AG) is detected in most organisms, but little is known about its metabolism and physiological roles. Our previous study demonstrated that Escherichia coli C600 synthesizes AG when glucose is exhausted in the medium and that it temporarily releases AG into and then takes it back from the medium, thus forming a sharp peak in AG concentration in the medium a few hours after reaching stationary growth phase. The present study demonstrates that when glucose is absent in the culture medium, E. coli C600 takes up and phosphorylates AG and releases a large portion of it back into the medium in the form of a phosphate ester. [U-13C]AG was added to the medium after the exhaustion of glucose and the resulting [U-13C]AG phosphate was partially purified by several steps of anion exchange chromatography and identified as AG 6-phosphate by 13C-NMR. The identity of the phosphate ester was also confirmed by GC-MS analysis after further purification.
环状多元醇1,5-脱水-D-葡萄糖醇（AG）在大多数生物体中都能被检测到，但对其代谢和生理作用却知之甚少。我们之前的研究表明，当培养基中的葡萄糖耗尽时，大肠杆菌C600会合成AG，并且它会将AG暂时释放到培养基中，随后又从培养基中摄取回来，从而在达到稳定生长期后的几个小时内在培养基中形成AG浓度的一个尖峰。本研究表明，当培养基中不存在葡萄糖时，大肠杆菌C600会摄取AG并将其磷酸化，然后将其中的一大部分以磷酸酯的形式释放回培养基中。在葡萄糖耗尽后，将[U-13C]AG添加到培养基中，所得的[U-13C]AG磷酸酯经过几步阴离子交换色谱法进行部分纯化，并通过13C-NMR鉴定为6-磷酸葡萄糖醇。在进一步纯化后，通过GC-MS分析也证实了磷酸酯的身份。
