Montero D, Tort L L, Izquierdo M S, Socorro J, Vergara J M, Robaina L, Fernandez-Palacios H
Instituto Canario de Ciencias Marinas, Gobierno de Canarias, Las Palmas, Canary Islands, Spain.
Rev Esp Fisiol. 1995 Dec;51(4):219-26.
To determine the hematological recovery after blood loss in gilthead sea bream juveniles, an experimental anemia was induced by caudal blood extraction. Seventy fish were used for experimental hemorrhage and another 35 were used as controls. Blood samples were taken after 0.5, 1, 2, 4, 8, 16 and 32 days in both control and anemic groups. After bleeding, besides a reduction in the direct hematic constants, a peak in the mean cell volume value, together with a decrease in mean cell hemoglobin concentration after bleeding, suggested erythrocyte swelling. The increase in MCH suggested the occurrence of hyperchromic erythrocytes. Recovery of RBC number started between 2 and 4 days after bleeding and seemed to be related to erythroblast release to circulation. Total recovery was completed after 8 days of bleeding. The recovery patterns for hematological parameters of sea bream are discussed in relation to applied work on this species.
为了确定金头鲷幼鱼失血后的血液学恢复情况，通过尾静脉采血诱导实验性贫血。70条鱼用于实验性出血，另外35条作为对照。在对照和贫血组中，分别在0.5、1、2、4、8、16和32天后采集血样。出血后，除了直接血液常数降低外，平均细胞体积值出现峰值，同时出血后平均细胞血红蛋白浓度降低，提示红细胞肿胀。平均血红蛋白含量的增加表明出现了高色素红细胞。红细胞数量的恢复在出血后2至4天开始，似乎与成红细胞释放到循环中有关。出血8天后完成完全恢复。结合对该物种的应用研究，讨论了金头鲷血液学参数的恢复模式。
