发育异常在人类溃疡性结肠炎中表现为核基质蛋白组成的改变。

Dysplasia expresses altered nuclear matrix protein composition in human ulcerative colitis.

作者信息

Izzo R S, Pellecchia C

机构信息

Department of Medicine, Nassau County Medical Center, East Meadow, New York 11554, USA.

出版信息

Biochem Mol Biol Int. 1996 Oct;40(3):521-6. doi: 10.1080/15216549600201093.

DOI:10.1080/15216549600201093

PMID:8908361

Abstract

We compared nuclear matrix protein compositions (NMPS) in colonic tissue from three different patient groups: 1) normal controls; 2) ulcerative colitis (UC); and 3) ulcerative colitis with dysplasia (UC-dysplasia). NMPS were separated by two-dimensional electrophoresis using isoelectrofocusing and SDS-PAGE. Six specific NMPS (59,500 Kd, pI 6.6 and 6.3; 49,250 Kd, pI 7.4; 33,750 Kd, pI 7.5; 20,000 Kd, pI 7.5; and 19,000 Kd, pI 6.3) were identified in UC-dysplasia that were not found in normal controls nor in UC colonic tissue. These observations suggest that NMPS may serve as important biochemical markers of dysplasia in UC.

摘要

我们比较了三组不同患者结肠组织中的核基质蛋白组成（NMPS）：1）正常对照；2）溃疡性结肠炎（UC）；3）伴有发育异常的溃疡性结肠炎（UC-发育异常）。使用等电聚焦和SDS-PAGE通过二维电泳分离NMPS。在UC-发育异常中鉴定出六种特定的NMPS（59,500 Kd，pI 6.6和6.3；49,250 Kd，pI 7.4；33,750 Kd，pI 7.5；20,000 Kd，pI 7.5；以及19,000 Kd，pI 6.3），而在正常对照和UC结肠组织中未发现。这些观察结果表明，NMPS可能是UC中发育异常的重要生化标志物。