结肠癌与发育异常中核基质蛋白组成的比较。

Comparison of nuclear matrix protein composition in colon cancer and dysplasia.

作者信息

Izzo R S, Pellecchia C

机构信息

Dept. of Medicine, Nassau County Medical Center, East Meadow, New York 11554, USA.

出版信息

Scand J Gastroenterol. 1998 Feb;33(2):191-4. doi: 10.1080/00365529850166932.

DOI:10.1080/00365529850166932

PMID:9517531

Abstract

BACKGROUND

Abnormal nuclear morphology associated with cancer may reflect changes in the proteins of the nuclear matrix.

METHODS

Nuclear matrix (NM) proteins were isolated from colonic tissue and analyzed by two-dimensional gel electrophoresis.

RESULTS

Several matrix proteins that were found in ulcerative colitis (UC) dysplasia (n = 5) and/or UC cancer (n = 4) were not identified in normal colonic tissue. UC dysplasia tissue showed three specific NM proteins with molecular masses of 49.2 kDa, 20.0 kDa, and 19.0 kDa, whereas 29.0-kDa and 32.0-kDa proteins were specific to UC cancer. Three proteins with 59.5-kDa (pI 6.3 and 6.6) and 33.75-kDa (pI 7.5) masses were common to both dysplasia and cancer tissue.

CONCLUSIONS

These data suggest that NM proteins may have a role in the transition of tissue towards the malignant phenotype.

摘要

背景

与癌症相关的异常核形态可能反映核基质蛋白的变化。

方法

从结肠组织中分离核基质（NM）蛋白，并通过二维凝胶电泳进行分析。

结果

在溃疡性结肠炎（UC）发育异常（n = 5）和/或UC癌（n = 4）中发现的几种基质蛋白在正常结肠组织中未被鉴定出来。UC发育异常组织显示出三种特定的NM蛋白，分子量分别为49.2 kDa、20.0 kDa和19.0 kDa，而29.0-kDa和32.0-kDa蛋白是UC癌特有的。两种发育异常组织和癌组织中都存在分子量为59.5-kDa（pI 6.3和6.6）和33.75-kDa（pI 7.5）的三种蛋白。