佛波酯诱导U937细胞分化后干扰素产生的增强。
Augmentation of interferon production after cell-differentiation of U937 cells by TPA.
作者信息
Indoh T, Shirakawa S, Kubota T, Yashiki T, Isogai E, Fujii N
机构信息
Department of Microbiology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
出版信息
Microbiol Immunol. 1996;40(9):675-9. doi: 10.1111/j.1348-0421.1996.tb01127.x.
Persistent infections with mumps virus were established in several human lymphoid cells of T-cell origin (Molt-4, TALL-1, and CCRF-CEM) and human monocyte cells (U937 and THP-1). 2',5'-Oligoadenylate synthetase (2-5AS) activity was demonstrated to be only slightly induced by interferon (IFN) or TPA (12-O-tetradecanoyl-phorbol-13-acetate) treatment in these cells. Treatment of the persistently infected cells with IFN or TPA did not stimulate an increase in the amount of synthetase mRNA. Induction of cell differentiation and augmentation of IFN production by TPA were demonstrated in U937 cells persistently infected with mumps virus (U937-MP). Similar results for IFN production were obtained from differentiated U937 cells. It is suggested that cell differentiation of U937 cells might be associated with the development of IFN inducibility.
腮腺炎病毒在几种T细胞来源的人淋巴细胞（Molt-4、TALL-1和CCRF-CEM）和人单核细胞（U937和THP-1）中建立了持续感染。在这些细胞中，2',5'-寡腺苷酸合成酶（2-5AS）活性仅在干扰素（IFN）或佛波酯（12-O-十四烷酰佛波醇-13-乙酸酯，TPA）处理后有轻微诱导。用IFN或TPA处理持续感染的细胞不会刺激合成酶mRNA量的增加。在持续感染腮腺炎病毒的U937细胞（U937-MP）中，TPA诱导了细胞分化并增强了IFN产生。从分化的U937细胞中获得了类似的IFN产生结果。提示U937细胞的分化可能与IFN诱导性的发展有关。
Zotero 插件