穿透性角膜移植术后非典型疱疹性上皮性角膜炎患者泪液中单纯疱疹病毒的聚合酶链反应检测
Polymerase chain reaction detection of herpes simplex virus in tear fluid from atypical herpetic epithelial keratitis after penetrating keratoplasty.
作者信息
Tei M, Nishida K, Kinoshita S
机构信息
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
出版信息
Am J Ophthalmol. 1996 Nov;122(5):732-5. doi: 10.1016/s0002-9394(14)70497-0.
PURPOSE
We investigated the efficacy of polymerase chain reaction (PCR) in diagnosing atypical epithelial keratitis after a penetrating keratoplasty (PKP) for herpetic corneal scarring.
METHODS
We examined three eyes of three patients with keratitis after a PKP for herpetic corneal scarring. The clinical presentation of the keratitis was atypical. DNA extracted from the tears was subjected to PCR and Southern blot hybridization.
RESULTS
Herpes simplex virus DNA was identified in all cases of presumptive recurrent herpetic keratitis.
CONCLUSION
Polymerase chain reaction is rapid and effective for diagnosing atypical recurrent herpetic keratitis.
目的
我们研究了聚合酶链反应（PCR）在诊断因疱疹性角膜瘢痕行穿透性角膜移植术（PKP）后非典型上皮性角膜炎中的疗效。
方法
我们检查了3例因疱疹性角膜瘢痕行PKP术后发生角膜炎患者的3只眼。角膜炎的临床表现不典型。从泪液中提取的DNA进行PCR和Southern印迹杂交。
结果
在所有疑似复发性疱疹性角膜炎病例中均鉴定出单纯疱疹病毒DNA。
结论
聚合酶链反应在诊断非典型复发性疱疹性角膜炎方面快速且有效。
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