Evaluation of a microspectrofluorimetric method or serum vitamin A.
作者信息
Sivakumar B
出版信息
Clin Chim Acta. 1977 Aug 15;79(1):189-95. doi: 10.1016/0009-8981(77)90477-6.
PMID:890951
Abstract
A microspectrofluorimetric method for vitamin A was compared with spectrophotometric and trifluoroacetic acid-colorimetric procedures. Values obtained by the fluorimetric method showed a high degree of correlation with those obtained by other standard procedures. However, it was found that the fluorimetric method yielded values which were several-fold higher than those obtained with the spectrophotometric method when sera from pregnant women were tested. The fluorescence from sera of pregnant subjects showed a triphasic decline even when the samples were stored at --20 degrees C.
摘要
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