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用薄层凝胶过滤免疫电泳法测定人支气管分泌物中IgA 7S/IgA 11S比值(作者译)

[Determination of the IgA 7 S/IgA 11 S ratio in human bronchial secretion by thin layer gel filtration immunoelectrophoresis (author's transl)].

作者信息

Laine A, Hayem A, Lebas J, Romon A

出版信息

Clin Chim Acta. 1977 Sep 15;79(3):541-8. doi: 10.1016/0009-8981(77)90174-7.

Abstract

After extraction by treatment with Ecteola-cellulose, IgA 11 S and IgA 7 S from human bronchial secretion were first separated by thin-layer gel filtration in Ultrogel AcA 3-4, and then were analyzed by crossed immunoelectrophoresis with an immune serum against alpha chain. Precipitation areas were measured separately. A correlation factor was necessary to express IgA 7 S in terms of IgA 11 S: it was calculated with standard curves made with serum and colostral IgA.

摘要

用ECTEOLA-纤维素处理提取后,人支气管分泌物中的IgA 11 S和IgA 7 S首先在Ultrogel AcA 3 - 4中通过薄层凝胶过滤分离,然后用抗α链免疫血清通过交叉免疫电泳进行分析。分别测量沉淀区。需要一个相关因子来根据IgA 11 S表示IgA 7 S:它是用血清和初乳IgA制作的标准曲线计算得出的。

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