Laine A, Hayem A, Lebas J, Romon A
Clin Chim Acta. 1977 Sep 15;79(3):541-8. doi: 10.1016/0009-8981(77)90174-7.
After extraction by treatment with Ecteola-cellulose, IgA 11 S and IgA 7 S from human bronchial secretion were first separated by thin-layer gel filtration in Ultrogel AcA 3-4, and then were analyzed by crossed immunoelectrophoresis with an immune serum against alpha chain. Precipitation areas were measured separately. A correlation factor was necessary to express IgA 7 S in terms of IgA 11 S: it was calculated with standard curves made with serum and colostral IgA.
用ECTEOLA-纤维素处理提取后,人支气管分泌物中的IgA 11 S和IgA 7 S首先在Ultrogel AcA 3 - 4中通过薄层凝胶过滤分离,然后用抗α链免疫血清通过交叉免疫电泳进行分析。分别测量沉淀区。需要一个相关因子来根据IgA 11 S表示IgA 7 S:它是用血清和初乳IgA制作的标准曲线计算得出的。