Bruno J G, Collard S B, Kuch D J, Cornette J C
Applied Research Associates, Tyndall Air Force Base, FL 32403, USA.
J Biolumin Chemilumin. 1996 Jul-Oct;11(4-5):193-206. doi: 10.1002/(SICI)1099-1271(199607)11:4/5<193::AID-BIO417>3.0.CO;2-2.
Low level intrinsic electrochemiluminescence (ECL) was induced from body fluids and homogenized tissues of oysters and several species of tunicates. No significant ECL was detected in human blood cell lysates, or bovine haematin, but minor ECL was observed in avian blood cell lysates. Both terrestrial grass and seagrass exhibited ECL, which is probably attributable to chlorophyll, since dead (brown) grass did not demonstrate ECL. It was postulated that organic-metal complexes in marine invertebrates were, at least in part, responsible for the intrinsic ECL, since such animals are known to be rich in organically bound metals. However, alternative biochemical mechanisms for the observed ECL, which do not involve metal chelates, are possible. Various metal ions were added to the invertebrate preparations to determine whether exogenous metals could enhance or inhibit the ECL reactions. Strongly oxidizing metal ions such as Ag+, Au+, Cu2+, Hg2+ and Sb2+ at > or = 100 ppm severely inhibited the intrinsic ECL response. No statistically significant ECL enhancement due to addition of metal ions was noted. ECL "profiles' were generated which demonstrated differences in the ECL responses of individual tunicate preparations to the presence of various exogenous metal ions. Differences in ECL profiles may represent differences in types or levels of endogenous metal chelates or other biochemical constituents. In addition, synthetic tunichromes (tunicate pigments) were analysed for ECL in the presence and absence of various added metal ions. One synthetic tunichrome isomer demonstrated a specific ECL interaction with Hg2+, while the other demonstrated broader ECL activity with several metal ions.
在牡蛎及几种被囊动物的体液和匀浆组织中诱导出了低水平的内源性电化学发光（ECL）。在人血细胞裂解物或牛血红蛋白中未检测到显著的ECL，但在禽血细胞裂解物中观察到了轻微的ECL。陆生草和海草均表现出ECL，这可能归因于叶绿素，因为枯草（褐色）未显示出ECL。据推测，海洋无脊椎动物中的有机金属络合物至少部分地是内源性ECL的原因，因为已知此类动物富含有机结合的金属。然而，观察到的ECL也可能存在不涉及金属螯合物的其他生化机制。向无脊椎动物制剂中添加了各种金属离子，以确定外源金属是否能增强或抑制ECL反应。浓度≥100 ppm的强氧化性金属离子如Ag+、Au+、Cu2+、Hg2+和Sb2+严重抑制了内源性ECL反应。未观察到因添加金属离子而导致的具有统计学意义的ECL增强。生成了ECL“图谱”，显示了各个被囊动物制剂对各种外源金属离子存在时的ECL反应差异。ECL图谱的差异可能代表内源性金属螯合物或其他生化成分的类型或水平差异。此外，分析了合成被囊色素（被囊动物色素）在添加和未添加各种金属离子情况下的ECL。一种合成被囊色素异构体与Hg2+表现出特定的ECL相互作用，而另一种则与几种金属离子表现出更广泛的ECL活性。
