犬播散性曲霉病：一项免疫组织化学研究

Disseminated aspergillosis in a dog: an immunohistochemical study.

作者信息

Pérez J, Mozos E, de Lara F C, Paniagüa J, Day M J

机构信息

Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Córdoba, Spain.

出版信息

J Comp Pathol. 1996 Aug;115(2):191-6. doi: 10.1016/s0021-9975(96)80041-7.

DOI:10.1016/s0021-9975(96)80041-7

PMID:8910747

Abstract

An immunohistopathological study of a case of disseminated aspergillosis in a 4-year-old female German shepherd dog was carried out to characterize the cellular infiltrate within the granulomatous lesions of various organs. Polyclonal antibodies specific for canine immunoglobulins IgG, IgM, IgA, complement C3c, human CD3 antigen (pan T-lymphocyte marker), human lysozyme and alpha-1-antitrypsin and a monoclonal antibody for myeloid/ histocyte antigen (MAC 387) (macrophage marker) were used. In the mycotic granulomata of the heart, liver, spleen and kidneys a correlation was found between the numbers of CD3+ T lymphocytes and lysozyme+ macrophages (r = 0.9944, P = 0.0056), and between the numbers of CD3+ T lymphocytes and MAC 387+ macrophages (r = 0.9943, P = 0.0057).

摘要

对一只4岁雌性德国牧羊犬的播散性曲霉菌病病例进行了免疫组织病理学研究，以描述各器官肉芽肿性病变内的细胞浸润情况。使用了针对犬免疫球蛋白IgG、IgM、IgA、补体C3c、人CD3抗原（全T淋巴细胞标志物）、人溶菌酶和α-1抗胰蛋白酶的多克隆抗体，以及针对髓样/组织细胞抗原（MAC 387）（巨噬细胞标志物）的单克隆抗体。在心脏、肝脏、脾脏和肾脏的霉菌性肉芽肿中，发现CD3+ T淋巴细胞数量与溶菌酶+巨噬细胞数量之间存在相关性（r = 0.9944，P = 0.0056），以及CD3+ T淋巴细胞数量与MAC 387+巨噬细胞数量之间存在相关性（r = 0.9943，P = 0.0057）。