Singh V K, Biswas S, Pandey C M, Agarwal S S
Department of Immunology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow, India.
Pathobiology. 1996;64(3):150-5. doi: 10.1159/000164029.
Several studies have shown a direct correlation between temperature and immune response. The effect of elevated temperature on cytotoxic effector functions is not well known. In the present study we have investigated the effect of elevated temperatures (38.5 and 40 degrees C) on cytotoxicity of lymphokine-activated killer cells (LAK), monocyte cytotoxicity, cytotoxic T lymphocytes (CTL) and tumor necrosis factor (TNF)-mediated cytotoxicity, and compared them with results at 37 degrees C. LAK cell cytotoxicity was inhibited significantly at 40 degrees C (p < 0.01) and there was no marked difference at 38.5 degrees C (p = 0.17) as compared to 37 degrees C when natural killer (NK)-sensitive K562 cells were used as target. When NK-resistant Raji cells were used as a target, cytotoxicity was inhibited at 40 degrees C (p = 0.33). Monocyte cytotoxicity was enhanced at 40 degrees C (p = 0.19) as well as at 38.5 degrees C (p = 0.38) as compared to 37 degrees C but differences were not statistically significant. Lipopolysaccharide (LPS) enhanced monocyte cytotoxicity. LPS-stimulated enhancement was further enhanced significantly at 40 degrees C (P < 0.05) and 38.5 degrees C (p < 0.05). Cytotoxicity of CTL was significantly inhibited at 38.5 compared to 37 degrees C (p < 0.0002). TNF-mediated cytotoxicity was significantly enhanced at 40 degrees C (p < 0.0004) and 38.5 degrees C (p < 0.001).
多项研究表明温度与免疫反应之间存在直接关联。高温对细胞毒性效应功能的影响尚不清楚。在本研究中，我们调查了高温（38.5摄氏度和40摄氏度）对淋巴因子激活的杀伤细胞（LAK）的细胞毒性、单核细胞细胞毒性、细胞毒性T淋巴细胞（CTL）以及肿瘤坏死因子（TNF）介导的细胞毒性的影响，并将其与37摄氏度时的结果进行比较。当使用对自然杀伤（NK）敏感的K562细胞作为靶细胞时，40摄氏度时LAK细胞的细胞毒性显著受到抑制（p < 0.01），与37摄氏度相比，38.5摄氏度时无明显差异（p = 0.17）。当使用对NK有抗性的Raji细胞作为靶细胞时，40摄氏度时细胞毒性受到抑制（p = 0.33）。与37摄氏度相比，40摄氏度（p = 0.19）以及38.5摄氏度（p = 0.38）时单核细胞细胞毒性增强，但差异无统计学意义。脂多糖（LPS）增强了单核细胞细胞毒性。LPS刺激的增强在40摄氏度（P < 0.05）和38.5摄氏度（p < 0.05）时进一步显著增强。与37摄氏度相比，38.5摄氏度时CTL的细胞毒性显著受到抑制（p < 0.0002）。TNF介导的细胞毒性在40摄氏度（p < 0.0004）和38.5摄氏度（p < 0.001）时显著增强。
