Bando Y, HIggins D A
Department of Pathology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
Immunology. 1996 Sep;89(1):8-12. doi: 10.1046/j.1365-2567.1996.d01-703.x.
The occurrence of mRNAs encoding mu, nu and nu(delta Fc) immunoglobulin heavy chains and lambda light chains in organs of duck embryos from 16 days of incubation and ducklings up to 74 days of age was assessed by Northern hybridization. The mu message was first detected in bursa of Fabricius and spleen at 16 days of incubation and in cervical lymph nodes at 23 days of incubation, but in other organs (bone marrow, buffy coat, Harderian gland, liver) not until 7 17 days after hatching; in general, the appearance of the lambda message paralleled that of mu. Messenger RNAs encoding one or both of the nu isoforms were first detected in cervical lymph nodes at 25 days of incubation, in spleen and bursa in 1-day-old ducklings, in Harderian gland, bone marrow and liver from 10 to 17 days post-hatching and in buffy coat from 46 days. In most organs, the nu(delta Fc) message was detected prior to the nu message and predominated during the experiment; Harderian gland expressed the nu(delta Fc) message exclusively. These results indicate that bursa of Fabricius, spleen and cervical lymph nodes play early roles in the development of B cells and the ontogeny of duck immunoglobulins while other lymphoid organs support the later differentiation of plasma cells, and that IgY and IgY(delta Fc) are probably not simultaneous products of the same plasma cells.
通过Northern杂交评估了从孵化16天的鸭胚胎到74日龄雏鸭的器官中编码μ、ν和ν（ΔFc）免疫球蛋白重链以及λ轻链的mRNA的出现情况。μ信息最早在孵化16天时在法氏囊和脾脏中检测到，在孵化23天时在颈淋巴结中检测到，但在其他器官（骨髓、血沉棕黄层、哈德氏腺、肝脏）中直到孵化后7至17天才检测到；一般来说，λ信息的出现与μ信息平行。编码一种或两种ν同种型的mRNA最早在孵化25天时在颈淋巴结中检测到，在1日龄雏鸭的脾脏和法氏囊中检测到，在孵化后10至17天在哈德氏腺、骨髓和肝脏中检测到，在46天时在血沉棕黄层中检测到。在大多数器官中，ν（ΔFc）信息在ν信息之前被检测到，并且在实验期间占主导；哈德氏腺仅表达ν（ΔFc）信息。这些结果表明，法氏囊、脾脏和颈淋巴结在B细胞发育和鸭免疫球蛋白个体发生中起早期作用，而其他淋巴器官支持浆细胞的后期分化，并且IgY和IgY（ΔFc）可能不是同一浆细胞的同时产物。
