Condliffe A M, Chilvers E R, Haslett C, Dransfield I
Department of Medicine, Rayne Laboratory, University Medical School Edinburgh, UK.
Immunology. 1996 Sep;89(1):105-11. doi: 10.1046/j.1365-2567.1996.d01-711.x.
Lung injury in a variety of disease states is critically dependent on neutrophil-mediated inflammatory responses. Neutrophil recruitment to sites of infection or tissue damage requires co-ordinated regulation of neutrophil adhesion and activation status. We have examined the effects of treatment of human peripheral blood neutrophils with priming agents [lipopolysaccharide (LPS). tumor necrosis factor-alpha (TNF-alpha) and platelet-activating factor (PAF)] upon expression of CD11a. CD11b. CD11c. CD35 and CD62-1 and CD11b function to assess whether subtle regulation of neutrophil adhesion potential accompanies augmented formyl-methionyl-leucyl-phenylalanine-stimulated superoxide production. We have found that there are differential effects of priming concentrations of these agents. For LPS. CD62L loss occurs in the absence of changes in CD11b, whereas for PAF. CD11b up-regulation occurs in the absence of detectable loss of CD62-L. However, for TNF-2, decreased expression of CD62-L occurs concomitantly with increased expression of CD11b. In addition, we have shown that priming agents augment CD11b functional activity in a manner that parallels the priming of the respiratory burst. Thus, priming agents may differentially regulate neutrophil adhesive capacity and data presented in this manuscript suggest that the increased effector cell function observed in primed cells may be associated with a distinct repertoire of potential adhesive interactions.
多种疾病状态下的肺损伤严重依赖于中性粒细胞介导的炎症反应。中性粒细胞募集到感染或组织损伤部位需要对中性粒细胞的黏附及激活状态进行协调调节。我们研究了用启动剂[脂多糖（LPS）、肿瘤坏死因子-α（TNF-α）和血小板活化因子（PAF）]处理人外周血中性粒细胞对CD11a、CD11b、CD11c、CD35、CD62-L表达及CD11b功能的影响，以评估在甲酰甲硫氨酰亮氨酰苯丙氨酸刺激的超氧化物生成增加时，中性粒细胞黏附潜能是否伴随细微调节。我们发现这些启动剂的不同浓度有不同作用。对于LPS，CD62L丢失而CD11b无变化；而对于PAF，CD11b上调而未检测到CD62-L丢失。然而，对于TNF-α，CD62-L表达降低同时CD11b表达增加。此外，我们还表明启动剂以与呼吸爆发启动平行的方式增强CD11b功能活性。因此，启动剂可能对中性粒细胞黏附能力有不同调节作用，本研究报告的数据表明，在启动细胞中观察到的效应细胞功能增强可能与潜在黏附相互作用的独特组合有关。
