人主动脉瓣同种异体移植物会引发供体特异性免疫反应。
Human aortic valve allografts elicit a donor-specific immune response.
作者信息
Hogan P, Duplock L, Green M, Smith S, Gall K L, Frazer I H, O'Brien M F
机构信息
Lions Human Immunology Laboratories, Brisbane, Australia.
出版信息
J Thorac Cardiovasc Surg. 1996 Nov;112(5):1260-6; discussion 1266-7. doi: 10.1016/S0022-5223(96)70139-3.
OBJECTIVE
The nature and magnitude of the immunologic response to implantation of human cryopreserved aortic valve allografts was investigated.
METHODS
Twenty aortic valve allograft recipients were investigated for donor-specific antibody and T-cell-mediated responses with serial flow cytometric and microlymphocytotoxic crossmatch assays and one-way mixed lymphocyte cultures.
RESULTS
Donor-specific immunoglobulin G antibodies to class I and II human leukocyte antigens were first detected in the serum of all aortic valve allograft recipients at 30 days after implantation and persisted in substantial amounts in all but one of the recipients at day 365. Recipient T-cell alloreactivity toward donor lymphocytes was significantly increased at day 30 compared with levels before and 10 days after operation.
CONCLUSIONS
Cryopreserved aortic valve allografts elicit a substantial allogeneic response in recipients. This alloreactivity may contribute to the observed morphologic changes in aortic valve allografts and eventual long-term deterioration of allograft function.
目的
研究人类冷冻保存主动脉瓣同种异体移植植入后的免疫反应性质和程度。
方法
对20名主动脉瓣同种异体移植受者进行了研究，通过系列流式细胞术和微量淋巴细胞毒性交叉配型试验以及单向混合淋巴细胞培养，检测供体特异性抗体和T细胞介导的反应。
结果
植入后30天，在所有主动脉瓣同种异体移植受者血清中首次检测到针对I类和II类人类白细胞抗原的供体特异性免疫球蛋白G抗体，除一名受者外，所有受者在365天时该抗体仍大量存在。与术前及术后10天相比，受者T细胞对供体淋巴细胞的同种异体反应性在第30天显著增加。
结论
冷冻保存的主动脉瓣同种异体移植在受者体内引发了显著的同种异体反应。这种同种异体反应性可能导致观察到的主动脉瓣同种异体移植形态学变化以及同种异体移植功能的最终长期恶化。
Zotero 插件