Zhang Y, Raud J, Hedqvist P, Fredholm B B
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Eur J Pharmacol. 1996 Oct 17;313(3):237-42. doi: 10.1016/0014-2999(96)00523-7.
Propentofylline is an atypical xanthine derivative that blocks adenosine uptake and has been shown to protect against ischemia-induced cerebral damage. We have studied the effect of propentofylline on recruitment of polymorphonuclear leukocytes during acute peritonitis induced by zymosan in mice. Following i.p. injection of zymosan, recruitment of polymorphonuclear leukocytes, reflected by myeloperoxidase activity in the peritoneal cavity, increased from 2 h onwards, peaked at 4 h and then decreased gradually. Propentofylline antagonized the zymosan-induced peritoneal myeloperoxidase accumulation in a concentration-dependent manner. This effect of propentofylline was counteracted by the non-selective adenosine receptor antagonist theophylline (50 mg/kg), and by the selective adenosine A2A receptor antagonists, 4-amino-8-chloro-1-phenyl-[1,2,4]-triazolo[4,3-a]quinoxaline (CP 66713) and 1,3-dipropyl-8-[3,4-dimethoxystyryl]-7-methylxanthine (KF 17387) (both at 2 mg/kg). The results indicate that propentofylline can reduce polymorphonuclear leukocyte recruitment in vivo and that this effect is related to an action on adenosine A2A receptors.
丙戊茶碱是一种非典型的黄嘌呤衍生物，它能阻止腺苷摄取，并且已被证明可预防缺血性脑损伤。我们研究了丙戊茶碱对酵母聚糖诱导的小鼠急性腹膜炎期间多形核白细胞募集的影响。腹腔注射酵母聚糖后，腹腔中髓过氧化物酶活性所反映的多形核白细胞募集从2小时起开始增加，在4小时达到峰值，然后逐渐下降。丙戊茶碱以浓度依赖的方式拮抗酵母聚糖诱导的腹腔髓过氧化物酶积累。丙戊茶碱的这种作用可被非选择性腺苷受体拮抗剂茶碱（50毫克/千克）以及选择性腺苷A2A受体拮抗剂4-氨基-8-氯-1-苯基-[1,2,4]-三唑并[4,3-a]喹喔啉（CP 66713）和1,3-二丙基-8-[3,4-二甲氧基苯乙烯基]-7-甲基黄嘌呤（KF 17387）（均为2毫克/千克）抵消。结果表明，丙戊茶碱可在体内减少多形核白细胞募集，且这种作用与对腺苷A2A受体的作用有关。
