Sakr M F, Abdel-Aal A N
Department of Surgery, Faculty of Medicine, University of Alexandria, Egypt.
Int Surg. 1996 Apr-Jun;81(2):180-3.
The putative hepatoprotective effect of cyclosporine A (CyA) against the hepatic injury associated with ischemia and reperfusion was studied in rats after a two-thirds hepatectomy following a 60-minute period of ischemia of the unresected liver. Animals were divided into three distinct groups of 10 rats each Group 1 (controls) received 0.5 ml saline solution intravenously (i.v.) while group 2 animals were injected with CyA (5 mg/kg) i.v., 24 hours before the induction of hepatic ischemia. The hepatic ATP content and serum levels of ALT and LDH were determined in each animal. Rats in group 3 were subjected to two-thirds hepatectomy only without the induction of ischemia. All controls died within 72 hours (group 1). Pretreatment of CyA improved the 7-day survival to 60% (p < 0.01). All group 3 animals survived through seven days. The improved survival seen in CyA pretreated animals as compared to controls was reflected by a restoration of hepatic ATP content and reduction in the serum levels of ALT and LDH postoperatively. Based on these results, it may be concluded that CyA ameliorates the hepatic injury associated with ischemia and reperfusion and allows the liver to recover and regenerate. Subsequently, survival is enhanced.
在未切除的肝脏缺血60分钟后进行三分之二肝切除的大鼠中，研究了环孢素A（CyA）对与缺血再灌注相关的肝损伤的假定肝保护作用。将动物分为三组，每组10只大鼠。第1组（对照组）静脉注射（i.v.）0.5 ml生理盐水，而第2组动物在诱导肝缺血前24小时静脉注射CyA（5 mg/kg）。测定每只动物的肝脏ATP含量以及血清ALT和LDH水平。第3组大鼠仅接受三分之二肝切除，不进行缺血诱导。所有对照组动物在72小时内死亡（第1组）。CyA预处理将7天生存率提高到60%（p < 0.01）。所有第3组动物存活了7天。与对照组相比，CyA预处理动物的生存率提高表现为术后肝脏ATP含量恢复以及血清ALT和LDH水平降低。基于这些结果，可以得出结论，CyA可改善与缺血再灌注相关的肝损伤，并使肝脏恢复和再生。随后，生存率提高。
