Oland L A, Müller T, Kettenmann H, Hayashi J
Division of Neurobiology, University of Arizona, Tucson 85721, USA.
J Neurosci Methods. 1996 Oct 21;69(1):103-12. doi: 10.1016/S0165-0270(96)00025-8.
Interactions among receptor neurons, glial cells and neurons intrinsic to the antennal lobe of the moth underlie the formation of olfactory glomeruli. To isolate these interactions, as well as to understand the effect of a variety of humoral agents on differentiation of the neurons and glia, we generate primary cultures of neurons or glia. These methods are described. In addition. we describe a protocol for producing slice preparations of the developing moth brain that we are using to study the biophysical and morphological development of glial cells. This technique allows us to examine a class of glial cells associated with the glomeruli that otherwise are nearly inaccessible using standard intracellular recording techniques. It also preserves the 3-dimensional arrangement of glia that may strongly influence the development of glomeruli.
蛾类触角叶内的受体神经元、神经胶质细胞和固有神经元之间的相互作用是嗅觉小球形成的基础。为了分离这些相互作用，并了解各种体液因子对神经元和神经胶质细胞分化的影响，我们培养了神经元或神经胶质细胞的原代培养物。本文描述了这些方法。此外，我们还描述了一种制备发育中蛾类大脑切片标本的方案，我们正在用该方案研究神经胶质细胞的生物物理和形态发育。这项技术使我们能够检查与嗅觉小球相关的一类神经胶质细胞，而使用标准的细胞内记录技术几乎无法检测到这些细胞。它还保留了可能对嗅觉小球发育产生强烈影响的神经胶质细胞的三维排列。
