Cao Y, Reece A, Russell S D
Department of Botany and Microbiology, University of Oklahoma, Norman 73019-0245, USA.
Zygote. 1996 May;4(2):81-4. doi: 10.1017/s096719940000294x.
Viable sperm cells of Nicotiana tabacum were isolated by the semi-vivo technique. After pollination, excised styles were floated, cut end immersed, in a solution of 15% sucrose with 0.01% boric acid and 0.03% Ca(NO3)2 at 27 degrees C in a growth chamber until pollen tubes emerged. After sperm cells were formed (at least 8 h after pollination) tubes were immersed in a 9% mannitol solution. In this solution, sperm cells are nearly ellipsoidal and retain viability for over 6 h.
