Zaccai M, Lipshitz H D
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Zygote. 1996 May;4(2):159-66. doi: 10.1017/s096719940000304x.
Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA 90, 2512-16, 1993; Yue & Spradling, Genes Dev. 6, 2443-54, 1992). We report the identification of two novel adducin isoforms: a 95 x 10(3) Mr form (ADD-95) and an 87 x 10(3) Mr form (ADD-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissue-types; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of Add-hts mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.
内收蛋白是一种细胞骨架蛋白，在体外可使F - 肌动蛋白成束，并控制F - 肌动蛋白/血影蛋白细胞骨架网络的组装。果蝇类内收蛋白（Add）基因座（也称为“胡里台绍”（hts））编码一类蛋白质，其中几种与哺乳动物内收蛋白同源（丁等人，《美国国家科学院院刊》90，2512 - 2516，1993；岳和斯普拉德林，《基因与发育》6，2443 - 2454，1992）。我们报告鉴定出两种新的内收蛋白异构体：一种95×10³ Mr的形式（ADD - 95）和一种87×10³ Mr的形式（ADD - 87）。我们对ADD - 95和ADD - 87在卵子发生和胚胎发生过程中的分布模式进行了详细分析。这些异构体在几种细胞和组织类型中共同表达；然而，只有ADD - 87存在于卵母细胞的中期到后期。在卵子发生的第9和10阶段，ADD - 87存在于整个卵母细胞皮质中，但从第11阶段开始仅在前极可检测到，这与Add - hts mRNA首先定位到皮质然后定位到卵母细胞前极相关。在卵子发生的第10阶段之前，ADD - 87在卵母细胞皮质中与F - 肌动蛋白和血影蛋白共定位，这与哺乳动物研究预测的在细胞骨架组装或功能中的可能作用一致。
