Yamagishi N, Miyakoshi J, Ohtsu S, Day R S, Takebe H
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Japan.
J Radiat Res. 1995 Dec;36(4):239-47. doi: 10.1269/jrr.36.239.
Effects of the introduction of the Simian virus 40 T-antigen (SV40 T-Ag) gene to cultured human cells were examined in relation to radiosensitivity. Two relatively radioresistant tumor cell lines (T98 and G361) became significantly radiosensitive after the introduction of SV40 T-Ag, whereas radiosensitive tumor cell lines did not show a change in radiosensitivity. In contrast, a human fibroblast cell line became radioresistant after SV40 T-Ag introduction. T98 cells which have a mutation at codon 237 in the p53 gene were unable to form a complex between p53 protein and SV40 T-Ag, whereas G361, which became radiosensitive by a SV40 T-Ag introduction, formed the complex. This indicates that the status of p53 is independent of the change in radiosensitivity in the cell lines studied.
研究了将猿猴病毒40 T抗原（SV40 T-Ag）基因导入培养的人细胞后对辐射敏感性的影响。两个相对抗辐射的肿瘤细胞系（T98和G361）在导入SV40 T-Ag后变得对辐射敏感，而辐射敏感的肿瘤细胞系在辐射敏感性上没有变化。相反，一个人成纤维细胞系在导入SV40 T-Ag后变得抗辐射。p53基因第237位密码子发生突变的T98细胞无法在p53蛋白和SV40 T-Ag之间形成复合物，而通过导入SV40 T-Ag变得对辐射敏感的G361细胞则形成了该复合物。这表明在所研究的细胞系中，p53的状态与辐射敏感性的变化无关。
