Johansen K M, Johansen J, Baek K H, Jin Y
Department of Zoology and Genetics, Iowa State University, Ames 50011, USA.
J Cell Biochem. 1996 Dec 1;63(3):268-79. doi: 10.1002/(SICI)1097-4644(19961201)63:3%3C268::AID-JCB2%3E3.0.CO;2-V.
Little is known about what determines the nuclear matrix or how its reorganization is regulated during mitosis. In this study we report on a monoclonal antibody, mAb2A, which identifies a novel nuclear structure in Drosophila embryos which forms a diffuse meshwork at interphase but which undergoes a striking reorganization into a spindle-like structure during pro- and metaphase. Double labelings with alpha-tubulin and mAb2A antibodies demonstrate that the microtubules of the mitotic apparatus co-localize with this mAb2A labeled structure during metaphase, suggesting it may serve a role in microtubule spindle assembly and/or function during nuclear division. That the mAb2A-labeled nuclear structure is essential for cell division and/or maintenance of nuclear integrity was directly demonstrated by microinjection of mAb2A into early syncytial embryos which resulted in a disintegration of nuclear morphology and perturbation of mitosis.
关于是什么决定了核基质，或者在有丝分裂期间其重组是如何被调控的，我们所知甚少。在这项研究中，我们报告了一种单克隆抗体mAb2A，它识别果蝇胚胎中的一种新型核结构，该结构在间期形成弥散的网络，但在前期和中期会经历显著的重组，形成纺锤体样结构。用α-微管蛋白和mAb2A抗体进行的双重标记表明，有丝分裂装置的微管在中期与这种mAb2A标记的结构共定位，这表明它可能在核分裂期间的微管纺锤体组装和/或功能中发挥作用。通过将mAb2A显微注射到早期合胞体胚胎中，直接证明了mAb2A标记的核结构对于细胞分裂和/或核完整性的维持至关重要，这导致了核形态的解体和有丝分裂的扰动。
