Wintour E M, Alcorn D, Butkus A, Congiu M, Earnest L, Pompolo S, Potocnik S J
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
Kidney Int. 1996 Nov;50(5):1624-33. doi: 10.1038/ki.1996.478.
Using reverse-transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry we investigated the ontogeny of renin, angiotensinogen and angiotensin converting enzyme (ACE) in the mesonephros at 27 and 41 days of gestation, and the metanephros at 41 and 64 days of gestation in ovine fetuses (term is 145 to 150 days). The volume and composition of fetal urine, stored as allantoic fluid were measured in 12 fetuses at 27 days, and 13 fetuses at 41 days. Renin, angiotensinogen and ACE were identified in both meso- and metanephroi at 41 days but not in the mesonephros at 27 to 30 days. Allantoic fluid volumes were 21 +/- 3 and 45 +/- 5 ml at 27 to 30 days and 41 days, respectively. This fluid was significantly different in composition to that of amniotic fluid or maternal plasma. The results suggest that the mesonephros can substantially modify its glomerular filtrate by 27 days of gestation, and can produce local angiotensin II by 41 days.
我们运用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，研究了妊娠27天和41天绵羊胎儿中肾以及妊娠41天和64天绵羊胎儿后肾中肾素、血管紧张素原和血管紧张素转换酶（ACE）的个体发生情况（足月为145至150天）。在27天的12只胎儿和41天的13只胎儿中测量了作为尿囊液储存的胎儿尿液的体积和成分。41天时，在中肾和后肾中均鉴定出肾素、血管紧张素原和ACE，但在27至30天的中肾中未鉴定出。27至30天和41天时，尿囊液体积分别为21±3毫升和45±5毫升。这种液体的成分与羊水或母体血浆的成分有显著差异。结果表明，中肾在妊娠27天时可对其肾小球滤液进行实质性改变，在41天时可产生局部血管紧张素II。
