Dalla Via L, Di Noto V, Siliprandi D, Toninello A
Dipartimento di Chimica Biologica, Università di Padova, Italy.
Biochim Biophys Acta. 1996 Oct 23;1284(2):247-52. doi: 10.1016/s0005-2736(96)00140-x.
Non-equilibrium binding of spermine to mitochondrial membranes is studied in rat liver mitochondria by applying a new thermodynamic treatment of ligand-receptor interactions (Di Noto, V., Dalla Via, L., Toninello, A. and Vidali, M. (1996) Macromol. Theory Simul. 5, 165-181). The presence on mitochondrial membranes of two spermine binding sites, both with monocoordination, is demonstrated. The calculated binding energy is characteristic for weak interactions. The treatment allows also to evaluate the variations of the molar fraction ratio of spermine bound to sites 1 and 2 as function of total bound spermine. The possible role of the two sites is discussed.
通过应用一种新的配体 - 受体相互作用热力学处理方法（迪诺托，V.，达拉·维亚，L.，托尼内洛，A.和维达利，M.（1996年）《高分子理论与模拟》5，165 - 181），在大鼠肝脏线粒体中研究了精胺与线粒体膜的非平衡结合。结果表明线粒体膜上存在两个精胺结合位点，两者均为单配位。计算得到的结合能是弱相互作用的特征。该处理方法还能评估结合到位点1和位点2的精胺摩尔分数比随总结合精胺的变化情况。文中讨论了这两个位点可能的作用。
