Lewin E
Epilepsia. 1977 Sep;18(3):349-55. doi: 10.1111/j.1528-1157.1977.tb04977.x.
The release of 14C-containing compounds from rat cortical slices prelabeled with 14C-adenine consisted largely of adenosine (6-7%), inosine (13-18%), and hypoxanthine (70-74%), with small amounts of nucleotides including cyclic AMP and adenine. This efflux was increased by both ouabain (0.1 mM) and veratridine (0.05 mM), the increment in released radioactivity consisting almost entirely of these three compounds. However, relatively more inosine than adenosine output was evoked by ouabain while the reverse was true with veratridine. Phenytoin partially reversed the effect of both depolarizing agents. After prelabeling, the efflux from astrocytoma cell cultures contained predominantly inosine (74%) and hypoxanthine (23%) with little adenosine. Ouabain increased the release of 14C-adenine derivatives, and this increase was diminished by phenytoin. Preliminary studies with neuroblastoma cell cultures have shown considerable variability in the composition of the effluent, with hypoxanthine the prevalent compound and almost no adenosine. Ouabain enhanced the efflux from these cells, and this effect was apparently reversed by phenytoin.
用¹⁴C -腺嘌呤预标记的大鼠皮质切片中含¹⁴C化合物的释放，主要由腺苷（6 - 7%）、肌苷（13 - 18%）和次黄嘌呤（70 - 74%）组成，还有少量包括环磷酸腺苷和腺嘌呤在内的核苷酸。哇巴因（0.1 mM）和藜芦碱（0.05 mM）均可增加这种流出，释放放射性的增加几乎完全由这三种化合物组成。然而，哇巴因引起的肌苷输出比腺苷相对更多，而藜芦碱的情况则相反。苯妥英可部分逆转两种去极化剂的作用。预标记后，星形细胞瘤细胞培养物的流出物主要含有肌苷（74%）和次黄嘌呤（23%），腺苷很少。哇巴因增加了¹⁴C -腺嘌呤衍生物的释放，而苯妥英可减少这种增加。对神经母细胞瘤细胞培养物的初步研究表明，流出物的成分有很大差异，次黄嘌呤是主要化合物，几乎没有腺苷。哇巴因增强了这些细胞的流出，而这种作用显然可被苯妥英逆转。
