嗜热绿菌橙色绿屈挠菌反应中心的结晶与X射线分析。

Crystallization and X-ray analysis of the reaction center from the thermophilic green bacterium Chloroflexus aurantiacus.

作者信息

Feick R, Ertlmaier A, Ermler U

机构信息

Max-Planck-Institut für Biochemie, Martinsried, Germany.

出版信息

FEBS Lett. 1996 Nov 4;396(2-3):161-4. doi: 10.1016/0014-5793(96)01101-5.

DOI:10.1016/0014-5793(96)01101-5

PMID:8914980

Abstract

The photochemical reaction center of Chloroflexus (Cf.) aurantiacus, a membrane bound pigment-protein complex, has been crystallized in the presence of monodisperse polyoxyethylene detergents. The crystals possessed a pronounced polymorphism. Three different crystal forms belonging to triclinic, monoclinic and orthorhombic space groups have been characterized by X-ray analysis. The triclinic crystal form, with unit cell dimensions of a = 88 A, b = 115 A and c = 151 A, diffracts up to 3.2 A in two directions and to 4.0 A in the third direction.

摘要

橙色绿屈挠菌（Cf.）的光化学反应中心是一种膜结合色素蛋白复合物，它已在单分散聚氧乙烯洗涤剂存在的情况下结晶。这些晶体具有明显的多晶型现象。通过X射线分析对属于三斜、单斜和正交空间群的三种不同晶体形式进行了表征。三斜晶体形式的晶胞尺寸为a = 88 Å，b = 115 Å和c = 151 Å，在两个方向上可衍射至3.2 Å，在第三个方向上可衍射至4.0 Å。