从基因组DNA凋亡切割过程中产生的兆碱基大小片段中直接切除50千碱基对的DNA片段。

Direct excision of 50 kb pair DNA fragments from megabase-sized fragments produced during apoptotic cleavage of genomic DNA.

作者信息

Sestili P, Cattabeni F, Cantoni O

机构信息

Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Italy.

出版信息

FEBS Lett. 1996 Nov 4;396(2-3):337-42. doi: 10.1016/0014-5793(96)01130-1.

DOI:10.1016/0014-5793(96)01130-1

PMID:8915015

Abstract

The DNA of U937 cells exposed to two different apoptotic stimuli, namely the cocktail H2O2/3-aminobenzamide (3AB) and etoposide, was analyzed using field inversion gel electrophoresis (FIGE) as well as programmable, autonomously controlled electrode electrophoresis (PACE). The results obtained indicate that FIGE is not appropriate for sizing apoptotic DNA fragments. PACE appears to be more accurate and reliable and the results obtained with this technique strongly suggest that the 50 kb DNA fragments are directly excised from Mb-sized DNA fragments without the intermediate cleavage of 200-300 kb products.

摘要

使用脉冲场凝胶电泳（FIGE）以及可编程自主控制电极电泳（PACE）分析了暴露于两种不同凋亡刺激（即过氧化氢/3-氨基苯甲酰胺（3AB）混合物和依托泊苷）的U937细胞的DNA。所得结果表明，FIGE不适用于凋亡DNA片段的大小测定。PACE似乎更准确可靠，用该技术获得的结果强烈表明，50 kb的DNA片段是直接从Mb大小的DNA片段上切除的，没有200 - 300 kb产物的中间切割过程。