Campbell B G, Bartholow S, Rosin E
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
Am J Vet Res. 1996 Nov;57(11):1627-30.
To determine whether the antibacterial activity of 6 mg of gentamicin/kg of body weight given SC once daily, is equivalent to the standard gentamicin dose of 2 mg/kg given SC every 8 hours.
Guinea pigs with infected thigh wound: 5 in an untreated control group and 12 in 6 and 2 mg/kg gentamicin treatment groups.
Guinea pigs were inoculated with 10(9) Escherichia coli in the thigh muscle. Gentamicin treatment (2 mg/kg, SC, q 8 h or 6 mg/kg, SC, q 24 h) was begun 4 hours after E coli inoculation and continued for 72 hours. Four hours after the last gentamicin treatment, all guinea pigs were euthanatized and the cranial thigh muscle containing the entire inoculum was removed. Colony-forming units were counted to determine the E coli concentration in each thigh.
Mean +/- SD log10 colony-forming units was 9.293 +/- 0.074 in the control group, 8.161 +/- 0.478 in the 2 mg/kg treatment group, and 7.796 +/- 0.182 in the 6 mg/kg treatment group. One-way ANOVA revealed a significant (P < 0.05) difference between the control group and both treatment groups, and between both treatment groups.
Bacterial killing did not differ between gentamicin given at a dosage of 6 mg/kg once daily, compared with 2 mg/kg every 8 hours in guinea pigs infected with E coli.
Gentamicin dosage regimens with high peak concentration and long dosing interval are as efficacious as divided dosage regimens. These data support the concept that once daily administration of gentamicin for treatment of E coli infection should be investigated clinically.
确定每日一次皮下注射6毫克庆大霉素/千克体重的抗菌活性是否等同于每8小时皮下注射2毫克/千克的标准庆大霉素剂量。
大腿伤口感染的豚鼠：未治疗的对照组5只，6毫克/千克和2毫克/千克庆大霉素治疗组各12只。
豚鼠大腿肌肉接种10(9) 大肠杆菌。在接种大肠杆菌4小时后开始庆大霉素治疗（2毫克/千克，皮下注射，每8小时一次或6毫克/千克，皮下注射，每24小时一次），持续72小时。最后一次庆大霉素治疗4小时后，对所有豚鼠实施安乐死，并取出包含整个接种物的大腿近端肌肉。计算菌落形成单位以确定每条大腿中的大肠杆菌浓度。
对照组的平均±标准差log10菌落形成单位为9.293±0.074，2毫克/千克治疗组为8.161±0.478，6毫克/千克治疗组为7.796±0.182。单因素方差分析显示对照组与两个治疗组之间以及两个治疗组之间存在显著差异（P<0.05）。
在感染大肠杆菌的豚鼠中，每日一次给予6毫克/千克庆大霉素与每8小时给予2毫克/千克庆大霉素的细菌杀灭效果无差异。
具有高峰浓度和长给药间隔的庆大霉素给药方案与分次给药方案一样有效。这些数据支持临床上应研究每日一次给予庆大霉素治疗大肠杆菌感染的概念。
