U6小核仁RNA的结构特征以及与其他剪接体成分的动态相互作用导致前体信使核糖核酸剪接。

Structural features of U6 snRNA and dynamic interactions with other spliceosomal components leading to pre-mRNA splicing.

作者信息

Forné T, Labourier E, Antoine E, Rossi F, Gallouzi I, Cathala G, Tazi J, Brunel C

机构信息

Institut de Génétique Moléculaire, UMR 5535, CNRS, Université de Montpellier II, France.

出版信息

Biochimie. 1996;78(6):436-42. doi: 10.1016/0300-9084(96)84750-x.

DOI:10.1016/0300-9084(96)84750-x

PMID:8915533

Abstract

In the spliceosome, the pre-mRNA, U2 and U6 snRNAs fold into a catalytic structure exhibiting striking similarities with domain V and VI of group II introns. Building of this tripartite structure implies that an evolutionary conserved base pairing between U4 and U6 snRNAs should be disrupted to allow potentially U6 catalytic residue to interact with U2 snRNAs and the pre-mRNA. The steps leading to U4/U6 disruption have been recently discovered and have been shown to involve a modification of the 3' end of U6 snRNA and the hnRNP C protein.

摘要

在剪接体中，前体mRNA、U2和U6小核RNA折叠成一种催化结构，与II类内含子的结构域V和VI表现出惊人的相似性。这种三方结构的形成意味着U4和U6小核RNA之间进化上保守的碱基配对应该被破坏，以使潜在的U6催化残基能够与U2小核RNA和前体mRNA相互作用。导致U4/U6破坏的步骤最近已被发现，并且已证明涉及U6小核RNA的3'末端和核不均一核糖核蛋白C蛋白的修饰。