Veicsteinas A, Belleri M, Cinquetti A, Parolini S, Barbato G, Molinari Tosatti M P
Department of Biomedical Sciences and Biotechnologies, University of Brescia, Italy.
Bioelectromagnetics. 1996;17(5):411-24. doi: 10.1002/(SICI)1521-186X(1996)17:5<411::AID-BEM9>3.0.CO;2-6.
The effects of intermittent exposure (2 h on/22 h off) to a 200 microT horizontal, sinusoidally oscillating (50 Hz) magnetic field were studied in 210 fertilized chicken eggs. Two hundred ten control eggs (sham-exposed) were incubated in the same chamber as the experimental eggs. Chick embryos were examined for developmental anomalies and maturity stage after 48 h of incubation. Immunohistochemical analysis of extracellular membrane components (laminin, fibronectin, and type IV collagen) were conducted on day 7 and histological examinations for malformations of brain, liver, and heart, on days 7, 12, and 18 of incubation. Furthermore, egg fertility and egg weights were evaluated on days 2, 7, 12, and 18. The investigation also measured the body weight of chickens for 90 days from hatching and included histological analysis of body organs. Each variable was investigated blind. Statistical comparison between exposed and sham-exposed values did not show significant differences in any of the variables investigated. Thus, it appears that the exposure of embryos to an intermittent 200 microT magnetic field at 50 Hz does not cause developmental anomalies, changes in maturity stage, alterations in distribution of extracellular membrane components, or malformations in the brain, liver, or heart. Moreover, there were no differences in body weight, morphology, or histology of central nervous system, liver, heart, or testis in 90-day-old chickens hatched from exposed in comparison to sham-exposed eggs.
在210枚受精鸡蛋中研究了间歇性暴露（2小时开启/22小时关闭）于200微特斯拉水平、正弦振荡（50赫兹）磁场的影响。210枚对照鸡蛋（假暴露）与实验鸡蛋在同一孵化箱中孵化。孵化48小时后检查鸡胚胎的发育异常和成熟阶段。在孵化第7天对细胞外膜成分（层粘连蛋白、纤连蛋白和IV型胶原）进行免疫组织化学分析，并在孵化第7天、12天和18天对脑、肝和心脏的畸形进行组织学检查。此外，在第2天、7天、12天和18天评估鸡蛋的受精率和重量。该研究还测量了雏鸡孵化后90天的体重，并对身体器官进行了组织学分析。每个变量的研究都是在盲态下进行的。暴露组和假暴露组数值之间的统计学比较在任何研究变量中均未显示出显著差异。因此，似乎胚胎暴露于50赫兹、间歇性200微特斯拉磁场不会导致发育异常、成熟阶段变化、细胞外膜成分分布改变或脑、肝或心脏畸形。此外，与假暴露鸡蛋孵化出的90日龄雏鸡相比，暴露组雏鸡在体重、形态或中枢神经系统、肝、心脏或睾丸的组织学方面没有差异。
