维甲酸对人上皮和纤维母细胞瘤细胞系中人类免疫缺陷病毒长末端重复序列的转录激活作用

Transcriptional activation of the human immunodeficiency virus long terminal repeat sequences by retinoic acid in human epithelial and fibroblast tumor cell lines.

作者信息

Zoumpourlis V, Ergazaki M, Spandidos D A

机构信息

Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.

出版信息

Int J Biol Markers. 1996 Jul-Sep;11(3):153-8. doi: 10.1177/172460089601100303.

DOI:10.1177/172460089601100303

PMID:8915710

Abstract

We employed a recombinant plasmid, pBHIV1, carrying the long terminal repeat (LTR) sequences of HIV-1 linked to the reporter chloramphenicol acetyl transferase (CAT) gene and to the aminoglycoside phosphotransferase (aph) gene as a selectable marker. We introduced pBHIV1 into human epithelial and fibroblast tumor cell lines (HeLa and MRCSV40TGR), and obtained stable geneticin-resistant HLHIV1-A and SVTGHIV1-A cells, respectively. The response to the retinoic acid was studied on the LTR regulated CAT activity in both cell lines. It was found that retinoic acid at a concentration of 1x10(-5) effects a 3.2 -fold increase in CAT expression compared to HIV LTR in HLHIV1-A, but requires a concentration of 5x10(-5) M to enhance this expression 4.6-fold in SVTGHIV1-A cells. These data show that retinoic acid may play a critical role in HIV-1 expression in human epithelial and fibroblast cell lines.

摘要

我们使用了一种重组质粒pBHIV1，它携带与报告基因氯霉素乙酰转移酶（CAT）基因以及作为选择标记的氨基糖苷磷酸转移酶（aph）基因相连的HIV-1长末端重复序列（LTR）。我们将pBHIV1导入人上皮和成纤维细胞瘤细胞系（HeLa和MRCSV40TGR），分别获得了稳定的对遗传霉素有抗性的HLHIV1-A和SVTGHIV1-A细胞。在这两种细胞系中，研究了视黄酸对LTR调控的CAT活性的反应。结果发现，在HLHIV1-A中，浓度为1×10⁻⁵的视黄酸使CAT表达比HIV LTR增加3.2倍，但在SVTGHIV1-A细胞中需要5×10⁻⁵ M的浓度才能使该表达增加4.6倍。这些数据表明，视黄酸可能在人上皮和成纤维细胞系中HIV-1的表达中起关键作用。