丝裂霉素C刺激大鼠和人成纤维细胞中人类免疫缺陷病毒长末端重复序列的表达。

Mitomycin C stimulates the expression of human immunodeficiency virus long terminal repeat sequences in rat and human fibroblasts.

作者信息

Zoumpourlis V, Spandidos D A

机构信息

Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.

出版信息

Biochem Pharmacol. 1993 Jul 6;46(1):178-81. doi: 10.1016/0006-2952(93)90363-2.

DOI:10.1016/0006-2952(93)90363-2

PMID:8347129

Abstract

We have employed a recombinant plasmid, pBHIV1, carrying the long terminal repeat (LTR) sequences of the human immunodeficiency virus-1 (HIV-1) linked to the reporter chloramphenicol acetyl transferase (CAT) gene and to the aminoglycoside phosphotransferase (aph) gene as a selectable marker. We have introduced pBHIV1 into rat 208F and human MRCSV40TGR fibroblasts and obtained stable geneticin resistant RFBHIV1-1 and SVTGHIV1-1 cells, respectively. Both transfectant cells express CAT activity from the HIV LTR promoter. The response to the antineoplastic drug mitomycin C was studied on the LTR regulated CAT activity in both cell lines. It was found that mitomycin C at 10 micrograms/mL concentration stimulates the expression of CAT from the HIV LTR 77-fold in rat RFBHIV1-1 and 3.1-fold in human SVTGHIV1-1 cells.

摘要

我们使用了一种重组质粒pBHIV1，它携带人类免疫缺陷病毒1型（HIV-1）的长末端重复序列（LTR），该序列与报告氯霉素乙酰转移酶（CAT）基因以及作为选择标记的氨基糖苷磷酸转移酶（aph）基因相连。我们已将pBHIV1导入大鼠208F和人MRCSV40TGR成纤维细胞，并分别获得了稳定的对遗传霉素有抗性的RFBHIV1-1和SVTGHIV1-1细胞。两种转染细胞均从HIV LTR启动子表达CAT活性。在这两种细胞系中，研究了抗肿瘤药物丝裂霉素C对LTR调控的CAT活性的影响。结果发现，浓度为10微克/毫升的丝裂霉素C可使大鼠RFBHIV1-1细胞中HIV LTR的CAT表达刺激77倍，在人SVTGHIV1-1细胞中刺激3.1倍。