Reese B E, Johnson P T, Baker G E
Neuroscience Research Institute, University of California at Santa Barbara 93106-5060.
J Comp Neurol. 1996 Nov 11;375(2):252-73. doi: 10.1002/(SICI)1096-9861(19961111)375:2<252::AID-CNE6>3.0.CO;2-1.
In the present set of studies, we have examined the site for the initiation of retinal maturation in the ferret. A variety of maturational features across the developing inner and outer retina were examined by using standard immunohistochemical, carbocyanine dye labelling, and Nissl-staining techniques, including 1) two indices of early differentiation of the first-born retinal ganglion cells, the presence of beta-tubulin and of neuron-specific enolase; 2) the receding distribution of chondroitin sulfate proteoglycans within the inner retina; 3) the distribution of the first ganglion cells to grow axons along the optic nerve; 4) the emergence of the inner plexiform layer; 5) the emergence of the outer plexiform layer and 6) the onset of synaptophysin immunoreactivity within it; 7) the differentiation of calbindin-immunoreactive horizontal cells; and 8) the cessation of proliferative activity at the ventricular surface. Although we were able to define distinct maturational gradients that are associated with many of these features of inner and outer retinal development (each considered in detail in this report), with dorsal retina maturing before ventral retina, and with peripheral retina maturing last, none showed a clear initiation in the region of the developing area centralis. Rather, maturation began in the peripapillary retina dorsal to the optic nerve head, which is consistent with previous studies on the topography of ganglion cell genesis in the ferret. These results make clear that the order of retinal maturation and the formation of the area centralis are not linked, at least not in the ferret.
在本系列研究中，我们研究了雪貂视网膜成熟起始的部位。我们运用标准免疫组织化学、羰花青染料标记和尼氏染色技术，检查了发育中的视网膜内外层的多种成熟特征，包括：1）最早生成的视网膜神经节细胞早期分化的两个指标，即β-微管蛋白和神经元特异性烯醇化酶的存在；2）硫酸软骨素蛋白聚糖在内层视网膜中的退缩分布；3）沿视神经生长轴突的第一批神经节细胞的分布；4）内网层的出现；5）外网层的出现；6）其中突触素免疫反应性的开始；7）钙结合蛋白免疫反应性水平细胞的分化；8）室表面增殖活动的停止。尽管我们能够确定与视网膜内外层发育的许多这些特征相关的明显成熟梯度（本报告中对每个特征都进行了详细考虑），即背侧视网膜先于腹侧视网膜成熟，周边视网膜最后成熟，但没有一个在发育中的中央凹区域显示出明确的起始。相反，成熟始于视神经乳头背侧的视乳头周围视网膜，这与之前关于雪貂神经节细胞发生地形学的研究一致。这些结果清楚地表明，视网膜成熟的顺序与中央凹的形成没有关联，至少在雪貂中是这样。
