Orrego F, Miranda R
Eur J Pharmacol. 1977 Aug 1;44(3):275-8. doi: 10.1016/0014-2999(77)90074-7.
Electrically induced release of 3H-noradrenaline from superfused rat brain cortex slices was completely inhibited by tetrodotoxin (1 microgram/ml) if 0.5 or 1 V stimuli were used, while inhibition was 97% with 2 V, and nil with 12 V stimuli. 20 mM calcium depressed noradrenaline release at all applied potentials, and shifted the release versus voltage curve to higher potentials. Manganese (5 mM) and D-600 (10-100 microgram/ml) also considerably inhibited release. This suggests that stimuli of up to 2 V induce transmitter release by exciting intracortical noradrenergic axons in their non-terminal regions.
如果使用0.5或1伏的刺激，河豚毒素（1微克/毫升）可完全抑制从灌流大鼠脑皮质切片中电诱导释放的3H-去甲肾上腺素，而使用2伏刺激时抑制率为97%，使用12伏刺激时则无抑制作用。20毫摩尔的钙在所有施加的电位下均抑制去甲肾上腺素的释放，并使释放与电压曲线向更高电位偏移。锰（5毫摩尔）和D-600（10 - 100微克/毫升）也显著抑制释放。这表明高达2伏的刺激通过兴奋皮质内去甲肾上腺素能轴突的非终末区域来诱导递质释放。
