Sakurai M, Aoki M, Abe K, Sadahiro M, Tabayashi K
Department of Thoracic and Cardiovascular Surgery, Tohoku University School of Medicine, Sendai, Japan.
Neurosci Lett. 1996 Oct 18;217(2-3):113-6.
The inductions of HSP72 and HSC73 mRNAs were investigated with Northern blot and immunohistochemical analyses for up to 7 days of the reperfusion after 5 or 15 min ischemia in rabbit spinal cord. Following 15 min ischemia, HSP72 mRNA was induced at 8 h of reperfusion, while HSC73 mRNA continuously remained at the control level. Immunoreactivity of HSP72 protein was slightly induced at 8 h of reperfusion selectively in motor neurons, and the majority of motor neuron showed selective cell death at 7 days of reperfusion. This study demonstrated selective induction of HSP72 mRNA and the protein in motor neuron cells that eventually showed selective delayed neuronal death after transient ischemia in the spinal cord. This phenomenon was not accompanied by HSC73 induction.
通过Northern印迹法和免疫组织化学分析，研究了兔脊髓在5或15分钟缺血后长达7天再灌注期间HSP72和HSC73 mRNA的诱导情况。缺血15分钟后，HSP72 mRNA在再灌注8小时时被诱导，而HSC73 mRNA持续维持在对照水平。HSP72蛋白的免疫反应性在再灌注8小时时在运动神经元中被选择性地轻微诱导，并且在再灌注7天时大多数运动神经元显示出选择性细胞死亡。本研究证明了在脊髓短暂缺血后，运动神经元细胞中HSP72 mRNA和蛋白的选择性诱导，最终这些运动神经元细胞出现选择性延迟性神经元死亡。这种现象未伴随着HSC73的诱导。
