Wells J M, Ventura R F, Eisenhauer P B, McKenna D C, Fine R E, Ullman M D
GRECC, ENRM VA Hospital, Bedford, MA 01730, USA.
Neurosci Lett. 1996 Oct 18;217(2-3):121-4.
Gangliosides, especially GM1, attenuate the in vivo damage caused by various neurotoxins. The chemically neutral inner ester of GM1 may be a better cytoprotective agent against some neurotoxins than the parent GM1 compound, because it may cross the blood-brain barrier (BBB) more easily than the anionic GM1. Using an in vitro bovine brain endothelial cell model of the BBB, we show the inner ester more readily transverse the tight junction barrier of this model than does GM1. Further, it is demonstrated that the GM1 inner ester is stable for several hours at pH values between 7.0 and 8.2 at 37 degrees C. Finally, the results illustrate that the BBB model may be useful for testing other gangliosides and their various derivatives for increased ability to cross the BBB.
神经节苷脂，尤其是GM1，可减轻各种神经毒素在体内造成的损伤。GM1的化学中性内脂可能比母体GM1化合物更能有效抵御某些神经毒素，因为它可能比带阴离子的GM1更容易穿过血脑屏障（BBB）。利用体外血脑屏障牛脑内皮细胞模型，我们发现内脂比GM1更容易穿过该模型的紧密连接屏障。此外，研究表明GM1内脂在37℃、pH值介于7.0至8.2之间时可稳定存在数小时。最后，结果表明血脑屏障模型可能有助于测试其他神经节苷脂及其各种衍生物穿过血脑屏障的能力是否增强。
