Carbone A, Gloghini A
Divisione di Anatomia Patologica, Istituto Nazionale di Ricovero e Cura a Carattere Scientifico, Aviano.
Pathologica. 1996 Jun;88(3):169-74.
Recent data have shown that CD40 antigen and its ligand (CD40L), and the CD26 molecule are differentially expressed among Hodgkin's disease (HD) (CD40+, CD26, CD40L), peripheral T-cell (PTCL) (CD40L+/-, CD40, CD26) and CD30 positive anaplastic large cell (ALC) (CD26+, CD40L) lymphomas. Since CD40 molecule is consistently detected on Reed-Sternberg (RS) cells and their morphologic variants with a distinctive staining pattern. CD40 expression provides a new potential tool for the identification of RS cells of HD in fixed and paraffin-embedded material. Furthermore, the combined application of anti-CD40 and anti-CD26 MoAbs may discriminate HD from morphologically and immunophenotypically related ALC lymphomas. In fact a CD26+/CD40 profile is restricted to CD30 positive ALC lymphomas. Finally, the combined application of anti-CD40 and anti-CD40L MoAbs provides additional significant features in differentiating HD from PTCL due to the constant expression of CD40+/CD40L phenotype in HD and its absence in PTCL, which in turn express CD40/CD40L+/- phenotype.
近期数据显示，CD40抗原及其配体（CD40L）以及CD26分子在霍奇金淋巴瘤（HD）（CD40阳性、CD26阴性、CD40L阴性）、外周T细胞淋巴瘤（PTCL）（CD40L阳性或阴性、CD40阴性、CD26阳性）和CD30阳性间变性大细胞（ALC）淋巴瘤（CD26阳性、CD40L阴性）中存在差异表达。由于在里德-斯腾伯格（RS）细胞及其形态学变异体上始终能检测到CD40分子，且具有独特的染色模式。CD40表达为在固定及石蜡包埋材料中识别HD的RS细胞提供了一种新的潜在工具。此外，抗CD40和抗CD26单克隆抗体的联合应用可将HD与形态学和免疫表型相关的ALC淋巴瘤区分开来。事实上，CD26阳性/CD40阴性的表型仅限于CD30阳性的ALC淋巴瘤。最后，抗CD40和抗CD40L单克隆抗体的联合应用在区分HD与PTCL方面提供了额外的重要特征，因为HD中CD40阳性/CD40L阴性表型持续表达，而PTCL中不存在这种表型，PTCL表达的是CD40/CD40L阳性或阴性表型。
